Four players 24 and under already have spots: forwards Larkin, 22, Andreas Athanasiou, 24, Tyler Bertuzzi 23, Anthony Mantha, 23.
Holland said two or three others likely will make the opening roster, but it depends on who seizes the job. It's uncertain whether the Red Wings will carry 14 forwards and seven defensemen, or 13 and eight.
"If two young defensemen make our team, maybe we'll carry eight defensemen," Holland said.
The Red Wings will give long looks in the preseason to forwards Evgeny Svechnikov, 21, Michael Rasmussen, 19, and Filip Zadina, 18, and defensemen Joe Hicketts, 22, Dennis Cholowski, 20, and Filip Hronek, 20.
Whoever doesn't make it could come up later in the season.
"Everybody obviously focuses in on opening day," Holland said. "But it's six-month season, and lots happens."