Barkov left a 4-0 loss to the Montreal Canadiens during the second period Saturday after taking a hit from defenseman Shea Weber. He had an MRI on Sunday.
"Day to day, we'll see," Panthers coach Joel Quenneville said following the morning skate Monday. "We felt last game when we lost him, it affects your power play, 5-on-5 important minutes and penalty killing. He plays big minutes, important minutes and obviously you're missing a very important piece but that's all part of it over the course of a season, so it will be a good test for us to play the right way and do the little things we need to do to have success."
Barkov has 54 points (16 goals, 38 assists) in 50 games this season. The Panthers captain has 399 points (151 goals, 248 assists) in 463 NHL games.
"It's a big adjustment," Florida forward Jonathan Huberdeau said. "Losing a player like that, he's our best player and so good in every situation but it's part of the game, we all have to step up. I think it's not going to be only one player. We all have to step up."
The Panthers (28-17-5), who have won six of their past seven games, are two points behind the Maple Leafs for third place in the Atlantic Division and two points out of the second wild card into the Stanley Cup Playoffs from the Eastern Conference.
NHL.com independent correspondent Dave McCarthy contributed to this story
