Buffalo is 0-13-2 since Feb. 25, the longest stretch without a win since the NHL implemented the shootout in 2005-06, ending tie games. The 2009-10 Carolina Hurricanes, 2010-11 New York Islanders and 2014-15 Sabres each lost 14 consecutive games.
"I guess anybody's confidence would be affected with the scenario," Buffalo coach Don Granato said. "They did continue to push through to the end. They skated hard, so there's no question everybody's confidence goes up and down."
Sidney Crosby and Zach Aston-Reese each had a goal and an assist for the Penguins (20-11-2), who lost three of their previous four after a six-game winning streak. Tristan Jarry made 26 saves.
Pittsburgh scored six goals total in its previous three games.
"We were just trying to stay in the moment," Penguins coach Mike Sullivan said. "We felt like we were playing a pretty solid game, and Buffalo was competing hard against us. It's just one of those situations where you try to focus in the now and not let your mind go to those types of places. We weren't really concerned.
"The dialogue on the bench was positive. Guys felt like they were generating scoring chances, and we were just trying to stay with it and score some goals."
Dustin Tokarski made 37 saves for the Sabres (6-21-4). It was his first NHL start since Dec. 21, 2015.
The teams play here again Thursday.
"Frustration has to be the first and the foremost [feeling]," Buffalo forward Curtis Lazar said. "As a team and as a whole, we have to understand playing skill-based hockey when you're in a slump is not going to cut it. ... It's on us. It's on us to right the ship here."
Evan Rodrigues tapped in a pass from Sam Lafferty near the right post to give the Penguins a 1-0 lead at 7:36 of the first period.
Rodrigues has scored twice in six games after an eight-game goal drought.
"I think guys just kind of take advantage of extra ice time, extra opportunity, and do the most they can to help the team win," Rodrigues said. "We obviously have a great leader (Crosby), and everyone follows his example."