Eric Fehr replaced Cullen as the fourth-line center at practice Tuesday, with left wing Chris Kunitz, and Scott Wilson and Tom Kuhnhackl splitting rushes at right wing.
The Penguins next play at the Montreal Canadiens on Wednesday (7:30 p.m. ET;
SN1, SNE, SNO, SNP, RDS, ROOT, NHL.TV).
Cullen has eight goals and 17 points in 43 games. He averages 13:16 of ice time per game, with 2:30 on the penalty kill, most among Penguins forwards.
Cullen, 40, is the third-oldest player in the NHL, after Florida Panthers forward Jaromir Jagr (44) and Arizona Coyotes forward Shane Doan, who is one month older than Cullen.
In 19 NHL seasons, Cullen has 243 goals and 675 points in 1,337 games.
