The forward was suspended by the NHL Department of Player Safety for Game 6, which the Blues lost 5-1 on Sunday, for an illegal check to the head of Bruins forward Marcus Johansson in Game 5.
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"Everyone's going to be ready," Barbashev said after practice Tuesday. "This is the last game of the season, and you've just got to give everything you've got. Game 7, there's nothing better than that. If you lose it, it hurts. If you win, it's even better feeling."
Barbashev, who has six points (three goals, three assists) in 24 Stanley Cup Playoff games, will play in his usual spot on the fourth line with center Oskar Sundqvist and Alexander Steen.
"Being a fourth line and playing big minutes, it's a huge part, it's a big role, especially when you play against the top players," Barbashev said. "It's huge. I'm really glad I got that opportunity because I want to get better. I want to get better at hockey, and so far it's been good. It's been a big learning process for me.